Abstract
Barium sulfate (BaSO4) is a material with high reflectance for preparing bifunctional sheets used in dry reagent chemical tests. In this study, bifunctional BaSO4 sheets with scattering power and high reflectance were prepared with BaSO4 microspheres sized 1.3~1.8 μm and cellulose acetate (CA). Factors such as the BaSO4 morphology, CA dosage, mixing time, surfactant, solid content and wet sheet thickness were investigated. Scanning electron microscopy (SEM), a dynamic contact angle test, light reflection detector and light reflection densitometer were employed to characterize the structure and properties of bifunctional BaSO4 sheets. The optimal conditions for preparing bifunctional BaSO4 sheets under natural drying conditions were as follows: mass ratio of CA to BaSO4 microspheres was 0.03:1; mixing acetone solution was used to form liquid stock puree; 35 μL Tween-80 was used to improve the hydrophilicity of bifunctional sheets; solid content was 54.9%; mixing 2 h then 450 µm thick sheets were cast on a glass plate using a film applicator. The light reflectance value of bifunctional BaSO4 sheets in the range of 400~800 nm was higher than 97%. Serum diffused in bifunctional BaSO4 sheets reacted in reagent sheets and formed uniform colorful spots. Considering the repeatability of spot proportion and light reflectance value, the sheet offered a uniform serum scattering power and good repeatability. Therefore, the bifunctional BaSO4 sheets are regarded as a promising material for dry chemical diagnostic reagents.
Highlights
In vitro diagnosis can be divided into two categories: wet chemical diagnosis and dry chemical diagnosis [1]
Dry chemical diagnosis refers to a method in which the liquid in a detected sample is used as a reaction medium, and the analyte directly reacts with a solid reagent solidified on the carrier [2]
(2) The reflection spectrometry detection results show that in the wavelength range of 400~800 nm, a bifunctional sheet prepared with BaSO4 is from 97% to over 98%, which is obviously higher than that of TiO2 bifunctional sheet
Summary
In vitro diagnosis can be divided into two categories: wet chemical diagnosis and dry chemical diagnosis [1]. The dry chemical diagnosis is a multi-layer structure which is composed of a bifunctional sheet, reagent sheet and support sheet. Bifunctional sheets have scattering power and high reflectance. Using a cellulose membrane as a scattering power sheet, because of its low reflectance and inability to preset the pre-reaction reagent, means the application range is narrow [11,12]. The bifunctional sheets prepared by BaSO4 microspheres with narrow particle size distribution are used in dry chemical diagnosis [15], which is conducive to form a wider response range, and can form uniform micron-level secondary voids between particles to filter and uniformly disperse serum. The bifunctional sheets with scattering power and high reflectance are prepared by BaSO4 microspheres with narrow particle size distribution. The structure and performance of the sheets are mainly characterized by scanning electron microscopy (SEM), a dynamic contact angle test, light reflection detector and light reflection densitometer
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