Preparation and pH Controlled Release of Fe3O4/Anthocyanin Magnetic Biocomposites.

Xizhi Jiang,Zhongzheng Gui,Mengyang Wang,Min Feng,Lei Xu,Nina Yan,Qingbao Guan,Min Wang

doi:10.3390/polym11122077

Abstract

Anthocyanins are a class of antioxidants extracted from plants, with a variety of biochemical and pharmacological properties. However, the wide and effective applications of anthocyanins have been limited by their relatively low stability and bioavailability. In order to expand the application of anthocyanins, Fe3O4/anthocyanin magnetic biocomposite was fabricated for the storage and release of anthocyanin in this work. The magnetic biocomposite of Fe3O4 magnetic nanoparticle-loaded anthocyanin was prepared through physical intermolecular adsorption or covalent cross-linking. Scanning electron microscopy (SEM), Dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD) and thermal analysis were used to characterize the biocomposite. In addition, the anthocyanin releasing experiments were performed. The optimized condition for the Fe3O4/anthocyanin magnetic biocomposite preparation was determined to be at 60 °C for 20 h in weak alkaline solution. The smooth surface of biocomposite from SEM suggested that anthocyanin was coated on the surface of the Fe3O4 particles successfully. The average size of the Fe3O4/anthocyanin magnetic biocomposite was about 222 nm. Under acidic conditions, the magnetic biocomposite solids could be repeatable released anthocyanin, with the same chemical structure as the anthocyanin before compounding. Therefore, anthocyanin can be effectively adsorbed and released by this magnetic biocomposite. Overall, this work shows that Fe3O4/anthocyanin magnetic biocomposite has great potential for future applications as a drug storage and delivery nanoplatform that is adaptable to medical, food and sensing.

Highlights

Anthocyanins are hydrophilic and water-soluble polyphenolic plant pigments and metabolites, which are one of the important antioxidants
We proposed an innovative methodology for the preparation of anthocyanin storage and release nanoplatforms, based on magnetite Fe3 O4 nanoparticles modified by amino polymers
A magnetic delivery system which could be stabilized by physical intermolecular or covalent cross-linking by altering temperatures or pH values is based on the surface modified by polymers cross-linking by altering temperatures or pH values is based on the surface modified by polymers [25]

Summary

Introduction

Anthocyanins are hydrophilic and water-soluble polyphenolic plant pigments and metabolites, which are one of the important antioxidants. They possess a wide range of pharmacological properties, such as antioxidant, antiaging, anti-inflammatory, antimicrobial and anti-cancer [1,2,3,4]. Current researches have demonstrated the strong antimicrobial effects of anthocyanins, e.g., inhibiting the growth of Listeria monocytogenes, Staphylococcus aureus, Salmonella enteritidis and Vibrio. Anthocyanins have the ability to induce HepG-2 cell apoptosis [7] and inhibit B16-F1 cell migration and Human umbilical vein endothelial cells (HUVECs) tube formation [8], showing strong anticancer activity. Encapsulation [12,13,14,15], composite [16,17,18,19] and group substitution [20,21,22,23,24]

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Results

Conclusion