Abstract
Objective To study the preparation and analyze the characteristics of 99Tcm-methoxy isobutyl isonitrile (MIBI) liposomes. Methods 99Tcm-MIBI liposomes were prepared through the ethanol injection-ultrasonication method. Liposome size and encapsulation efficiency were determined, and ultrasonication duration and soybean lecithin-to-cholesterol mass ratio were optimized to observe their effects on the stability of 99Tcm-MIBI liposomes. Results When the mass concentration of 99Tcm-MIBI was 0.01 mg/mL, the average particle size was (171.4±25.2) nm, and the average encapsulation efficiency was (8.5±1.3)%. The optimal preparation conditions consisted of the mass ratio of soy lecithin to cholesterol of 4:1, the 99Tcm-MIBI mass concentration of 0.01 mg/mL, and ultrasonication duration of 5 min. Electron micrograph images revealed that after the first day, particles reduced in size and appeared regular and uniform in shape. The particles appeared completely spherical or elliptical. Particle sizes and shapes negligibly changed after 30 d. Conclusion 99Tcm-MIBI liposomes with small particle size, high encapsulation efficiency, and stable entrapment efficiency can be obtained through the ethanol-ultrasonication technique. Key words: Liposomes; Technetium Tc 99m Sestamibi; Isotope labeling
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