Preparation and Characteristic of Medium-chain Fatty Acid Liposome

Abstract

Film-dispersion-dynamic high-pressure micro-fluidizer (Film dispersion-DHPM) method and DHPM-freezethawing method were used to prepare medium-chain fatty acid (MCFA) liposome with lecithin and cholesterol as the membrane materials, and MCFA as the core material. Encapsulation efficiency (EE) and average particle size of MCFA liposome were chosen to evaluate both preparation methods. The optimal preparation conditions of MCFA liposome using Film dispersionDHPM method were lipid content of 8% (m/V), lecithin-cholesterol ratio of 5:1 (m/m), treatment pressure of 140 MPa and repeated treatment number of 4. The optimal preparation conditions of MCFA liposome using DHPM-freeze-thawing method were freezing time of 0.5 h, maltose as the cryoprotectant content of 3% (m/V) and thawing temperature of 40 ℃. Under the optimal preparation conditions, the average diameter and EE of MCFA liposome prepared by Film-dispersion-DHPM method were 87.1 nm and (66.5 ± 3.1)%; MCFA liposome prepared by DHPM-freeze-thawing method exhibited a relative higher average diameter and lower EE, which were 110.1 nm and (53.5 ± 4.9)%, respectively. The stability of MCFA liposome was explored by leakage kinetics. Results exhibited that MCFA liposome prepared by Film dispersion-DHPM method and DHPM-freezethawing method had good stability. Therefore, both methods can be used to prepare MCFA liposome.