Preliminary Study on Whole Blood Preserving at Room Temperature

Abstract

Objective To research quality changes on plasma and red blood cell (RBC) separated from whole blood preserved at a higher temperature and a longer period.Methods In May 2012,a total of 10 bags of 200 mL whole blood which collected from 10 cases of healthy blood donors in our blood center were included in this study.All samples were separated into aliquot bags,then be divided parallel into two groups,the control group (n=10) was reserved at 22℃ for eight hours,the study group (n=10) was reserved at 22℃ for twenty four hours.All the whole blood bags of each group were prepared into frozen plasma and packed RBC.Frozen plasma were stored frozen and packed RBC were preserved at 4℃ for 35 d.Coagulation factors of frozen plasma and adenosine triphosphate (ATP),pH,free hemoglobin (FHb),K+of packed RBC were tested.Results In study group,FⅧ of frozen plasma significantly decreased (t=6.459,P＜0.05).The ATP and pH value of packed RBC showed comparable lower on the first day and seventh day,the potassium concentration rose greatly on the fourteenth day,the FHb value had an upward tendency but not a significant difference.Conclusions Whole blood preserving at 22℃ for twenty four hours was not appropriate for preparation of fresh frozen plasma,its adverse effects on packed RBC had reached acceptable levels,a shorter preserving period of packed RBC on this condition was suggested. Key words: whole blood; preserve; quality