Preliminary Phytochemical Screening on the Glycosides of Leaf Extracts of Medicinally Important Plant Holoptelea integrifolia (roxb.) Planch Using High Performance Thin Layer Chromatography

Abstract

Objective: This study was conducted to identify the glycosides from petroleum ether and methanol extracts of medicinally and economically useful leaves of Holoptelea integrifolia (Roxb.) planch using high-performance thin layer chromatography (HPTLC) technique. Materials and Methods: Preliminary phytochemical screening was carried out and HPTLC studies were carried out. Camag HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win computer-aided transcription system software (1.4.6 Camag) with the help of tungstant lamp. Results: Preliminary phytochemical screening of petroleum extract of H. integrifolia showed the presence of steroids, terpenoids, alkaloids, glycosides, flavonoids, proteins, tannins and carbohydrates, while methanolic extract of H. integrifolia showed the presence of steroids, alkaloids, flavonoids, proteins, and carbohydrates. HPTLC fingerprinting of glycosides of petroleum ether extract of leaf revealed four polyvalent phytoconstituents (4 peaks) and corresponding ascending order of retention factor (Rf) values in the range of 0.37-0.82. while methanol extract of leaf showed ten polyvalent phytoconstituents (10 peaks) and corresponding ascending order of Rf values in the range of 0.20-0.83. Conclusions: With the above Rf values and preliminary phytochemical analysis we have concluded the presence of glycosides in both the extracts. The present study revealed that the plant H. integrifolia is a good source of phytochemicals.