Preliminary Phytochemical Screening and HPTLC Fingerprinting of Leaf Methanolic Extract of Spermacoce hispida

Abstract

The main objective of the present work to established the fingerprint profile of S.hispida using high performance thin layer chromatography (HPTLC) technique. Preliminary phytochemical screening was done and HPTLC studies were carried out. The scanning was performed using 'CAMAG' densitometer scanner 3V equipped with CATS3 software, at a wavelength of 400 and 254 nm using deuterium lamp.Preliminary phytochemical screening of the methanolic extract S.hispida showed the presence of alkaloids, carbohydrates, phenols, tannins, flavanoids, gums and mucilage. HPTLC finger printing of S.hispida methanolic extract showed best results in Toluene: Ethyl Acetate: Formic acid: 5:4:1 solvent system. After scanning and visualizing the plates in absorbance mode at 254nm, 366 nm and visible light range (400-600nm after spraying with anisaldehyde sulphuric acid reagent) best results were shown at 400nm. The Rf values ranged from 0.06 to 0.99. The result showed that out of 15 components, the component with Rf values 0.95, 0.82 were found to be more predominant as the percentage area is more with 28.85% and 12.91% respectively.