High performance thin layer chromatography profile studies on the alkaloids of Albizia lebbeck

Abstract

ObjectiveTo establish alkaloids profile for the medicinally important plant Albizia lebbeck (A. lebbeck) using high performance thin layer chromatography (HPTLC). MethodsPreliminary phytochemical screening was carried out by Harborne method. HPTLC studies were followed by Harborne and Wagner et al method. Ethyl acetate-methanol-water (100:13.5:10) was employed as mobile phase for alkaloids. The developed plate was sprayed with Dragendorff's reagent followed by 10% sodium nitrite reagent as spray reagent and dried at 100 °C in hot air oven for 3 min. The plate was photo-documented at UV 366 nm and daylight using photo-documentation chamber. ResultsAlkaloids, steroids, terpenoids, flavonoids, saponins and glycosides present in the petroleum ether, ethyl acetate and methanolic extracts of A. lebbeck leaves. The petroleum ether extracts of A. lebbeck leaves displayed the presence of 10 types of alkaloids with 10 different Rf values ranging from 0.02 to 0.85. The ethyl acetate extract of A. lebbeck leaves illustrated the presence of 5 different types of alkaloids with 5 different Rf values ranging from 0.09 to 0.84. The methanolic extract of A. lebbeck leaves demonstrated the presence of 4 different types of alkaloids with 4 different Rf values with range from 0.02 to 0.79. Maximum number (10) of alkaloids has been observed in petroleum ether followed by ethyl acetate (5) extracts of the leaves of A. lebbeck. ConclusionsIn the present study we observed various alkaloids profile of the medicinally important plant using HPTLC. This profile can be used for the identification of the medicinally important plants from the adulterant. Further, separation and characterization of the bioactive compound (principles) from the plants is to be evaluated and reported in near future.