Preliminary Phytochemical Investigation and Eff ect of Cucurbita maxima Seeds Oil on Burn Wound Healing in Iraq

Abstract

Cucurbita maxima seeds oil were extracted by hot and cold method, identifi ed their phytochemical components as it contains alkaloids, fl avonoids, saponins and tannins and analyzed to explain its ability to heal burn wound. Male albino 32 Sprague- Dawley rats were divided into four rats in each cage which included a positive control group (Mebo ointment): two rats for fi rst-degree burn and two for the second one, negative control group: two rats for fi rst-degree burn and two for the second one, cold method: 100% concentration with two rats for fi rst-degree burn and two for the second one and hot method: 100, 75, 50, 25 and 10% concentration with two rats for fi rst-degree burn and two for the second one for each concentration. The results showed that the extracts of C. maxima seeds oil at fi rst and second-degree burn by the cold method were not effi cient so it could neglect where it compared to the hot procedure at the same time the concentrations 100, 75, 50, 25, and 10% were signifi cant as LSD5% were more than 0.01 when compared with the negative standard, while when compared with the positive standard 100 and 75% were signifi cant while 25, 10% were insignifi cant and 50% had the same activity as the positive standard. Also, there is a signifi cant diff erence between concentration at fi fth week; 100 and 75% has the largest signifi cant diff erences which is 0.01 among the other concentrations. At second-degree burn we notice that all the concentrations 100, 75, 50, 25, and 10% were signifi cant as LSD5% were more than 0.01 when compared with the negative control, while when compared with the positive control 100 and 75% were signifi cant while 25, 10% were insignifi cant and 50% had the same activity as the positive standard. Also there is a signifi cant diff erence between concentrations at fi fth week, 75% has the largest signifi cant diff erence among the other concentrations. As a fi nal result the concentration 100 and 75% were the best concentration in the treatment of burn wound as the 100% contained a large amount of extract and 75% contained a suffi cient quantity of extract in addition to the ointment base vaseline which aid in keeping the skin wet enough to avoid shrinkage of it and help in the rehabilitation of skin epithelial cells. This study of C. maxima seeds oil is the fi rst study in the healing of burn wound on rats in Iraq