Abstract
Objective: The present study was designed with the aim of preliminary phytochemical evaluation and spectral characterization phytochemicals present in the extracts of whole plant Argyreia imbricata (Rox) Sant and Patel.
 Methods: The whole plant A. imbricata was collected, authenticated, and dried in the shade for powdering in mechanical grinder. The powdered plant material was extracted with Soxhlet apparatus using different solvents, and the dried extract obtained was subjected to preliminary phytochemical evaluation, and then column chromatography separation and the separated fractions were subjected to thin-layer chromatography (TLC) separation. Based on the yield in TLC, the selected compounds were subjected to carbon-13 nuclear magnetic resonance, proton NMR (1H NMR), and mass spectroscopic evaluation.
 Results: In the preliminary phytochemical evaluation, the presence of alkaloids, glycosides, phenolic compounds, flavonoids, carbohydrates, saponins, sterols, and terpenoids was found in different extracts. In column chromatography separation, totally 650 fractions were collected, and four compounds were isolated with the help of TLC. Among the four, two compounds were selected for spectral evaluation based on the yield obtained. The selected compounds were identified and named as, 3-ethyl-5-(hydroxymethyl) phenol and methyl 4-hydroxybenzoate with the help of spectral evaluation.
 Conclusion: The outcome of this study is beneficial, and further investigation in the future directed toward the detailed pharmacological screening of the extracts may give more valuable results.
Highlights
IntroductionMedicinal plants are commonly distributed all over the world, but, notably in tropical countries
It is well known that the therapeutic usage of plant sources is time immemorial
The present study focused on the preliminary phytochemical screening of the plant A. imbricata and the characterization of active constituents by different spectral analysis
Summary
Medicinal plants are commonly distributed all over the world, but, notably in tropical countries. According to the World Health Organization, plants are one of the best sources for a variety of drugs, and approximately 25% of present-day medicines are derived directly or indirectly from higher plants [1,2,3]. Usage of medicinal plants in Asian countries indicates a longstanding tradition of human relationships with the environment. These medicinal plants contain a wide range of bioactive compounds that include alkaloids, flavonoids, tannins, and phenolic compounds which can be used to treat numerous chronic as well as infectious diseases [4,5,6,7]
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