Pregnancy-induced modulation of calcium mobilization and down-regulation of Rho-kinase expression contribute to attenuated vasopressin-induced contraction of the rat aorta

Abstract

Normal pregnancy is characterized by attenuated vascular reactivity to a variety of contractile agonists and this, in part, has been attributed to increased circulating vasodilators and/or impaired Ca 2+-influx through L-type Ca 2+-channels. Our hypothesis in this study was that reduced Ca 2+-dependent (influx) and Ca 2+-independent (involving the RhoA/Rho-kinase pathway) mechanisms contributed to attenuated vasopressin-induced contraction of the pregnant rat aorta. AVP (10 − 10 − 3 × 10 − 7 M) induced concentration-dependent contraction of aortic ring segments from nonpregnant and pregnant rats with no significant change in p D 2 values (8.53 ± 0.11 and 8.33 ± 0.18 in nonpregnant and pregnant rats, respectively). The maximum response was however significantly reduced in aorta segments from pregnant rats. Nifedipine (10 − 6 M) significantly inhibited AVP-induced contraction in artery segments from nonpregnant but not pregnant rats indicating a reduced role for Ca 2+-influx through L-type Ca 2+-channels in AVP-induced contractions of the pregnant rat aorta. Western blot analysis revealed the expression of ROCK-1 and ROCK-II isoforms in aorta segments from both groups. There was a significant reduction in the expression of ROCK-1 and ROCK-II isoforms in aortic tissues from pregnant rats. This is consistent with the reduced potency of Y-27632 in inhibiting AVP (10 − 7 M) induced contraction in aorta segments from pregnant rats. It was concluded that pregnancy-induced attenuated vascular response to AVP was due to decreased Ca 2+-influx through L-type Ca 2+-channels and decreased sensitization of the contractile myofilaments to Ca 2+.