Pregnancy‐induced down‐regulation of rho‐kinase expression contributes to attenuated vasopressin‐induced contraction of the rat aorta

Abstract

Normal pregnancy is characterized by attenuated vascular reactivity to a variety of contractile agonists and this, in part, has been attributed to increased circulating vasodilators and/or impaired Ca2+-influx through L-type Ca2+-channels. Our main objective in this study was to investigate the possible role of the RhoA/Rho-kinase pathway in attenuated vasopressin-induced contraction of the pregnant rat aorta. AVP (10−10–3x10−7 M) induced concentration-dependent contraction of aortic ring segments from nonpregnant and pregnant rats with no significant change in pD2 values (8.53 ± 0.11 and 8.33± 0.18 in nonpregnant and pregnant rats, respectively). The maximum response was however significantly reduced in aorta segments from pregnant rats. Nifedipine (10−6M) significantly inhibited AVP-induced contraction in artery segments from non-pregnant but not pregnant rats indicating impaired Ca2+-influx through L-type Ca2+-channels in pregnant rat aorta. Western blot analysis revealed the expression of ROCK-1 and ROCK-II isoforms in aorta segments from both groups. There was a significant reduction in the expression of ROCK-1 and ROCK-II isoforms in aortic tissues from pregnant rats. This is consistent with the reduced potency of Y-27632 in inhibiting AVP (10-7 M) induced contraction in aorta segments from pregnant rats. It was concluded that pregnancy-induced attenuated vascular response to AVP was due to decreased Ca2+-influx through L-type Ca2+-channels and decreased sensitization of the contractile myofilaments to Ca2+. Supported in part by the College of Graduate Studies