For the simultaneous preconcentrations of Cu(II) and Mn(II), a novel preconcentration technique was developed and described. Bacillus cereus loaded magnetic ɣ-Fe2O3 nanoparticles were prepared and used as support materials on solid-phase extraction procedure. Important experimental parameters were investigated in details and pH 6.0, 3 mL min−1 of flow rate, 5 mL of 1 mol L−1 of HCl as eluent, 200 mg of biomass, and 200 mg of magnetic ɣ-Fe2O3 nanoparticles as support material was found as the best conditions. The preconcentrations factor were found to be 80 for Cu (II) and Mn(II). It was confirmed by the results that SPE columns could be used in 32 cycles. The LOD values calculated for Cu (II) and Mn (II) were 0.09 and 0.08 ng mL−1, respectively. The RSD values found were less than 3.4%. The extraction recoveries were achieved as higher than 98%. The biosorption capacities of Cu (II), and Mn (II) were 26.0 mg g−1, 30.3 mg g−1 respectively. The approach devised for analyzing analyte concentrations in food samples proved to be successful.

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