Potential Hepatoprotective Effect of Cheatomorpha gracilis extract against High Fat Diet (HFD)-Induced Liver Damage, and its characterization by HPLC.

N Brahmi,A Elleuch,A El Feki,H Talarmin,M Ben Ali,B Albogami,M S Allagui,T Saif,A Hedfi,A Feriani,I A Ismail

doi:10.1590/1519-6984.247102

Abstract

The current investigation was carried out to estimate the protective effect of aqueous extract of Cheatomorpha gracilis (AEC) against High fat Diet (HFD) induced liver damage in mice. The results of the in vitro study showed that AEC have higher antioxidant capacities in the DPPH and hydroxyl radical-scavenging assays. Indeed, many phenolic compounds (gallic acid, quercetin, naringenin, apigenin, kaempferol and rutin) were identified in the AEC. In the animal studies, during 6 weeks, HFD promoted oxidative stress with a rise level of malonaldehyde (MDA), protein carbonyls (PCOs) levels and a significant decrease of the antioxidant enzyme activities such as superoxide dismutase, catalase and glutathione peroxidase. Interestingly, the treatment with AEC (250 mg/kg body weight) significantly reduced the effects of HFD disorders on some plasmatic liver biomarkers (AST, ALT and ALP) in addition to, plasmatic proteins inflammatory biomarkers (α2 and β1 decreases / β2 and γ globulins increases). It can be suggest that supplementation of MECG displays high potential to quench free radicals and attenuates high fat diet promoted liver oxidative stress and related disturbances.

Highlights

In today’s modern lifestyle, a rise in dietary fat intake, 2
Our data shown that yield extraction was varied between 0.96% and 13.44% depending on the extraction solvent with the following order: water > ethanol-water (70v-30v) > methanol > ethyl acetate > ethanol > dichloromethane
Our findings shown that the administration of High fat Diet (HFD) induced a severe liver injury as manifested by a significant rise in the activities of ALT, AST and alkaline phosphatase (ALP) in mice plasma

Summary

Introduction

In today’s modern lifestyle, a rise in dietary fat intake, 2. Material and Methods important changes in our food habits and a decline in physical action represented by rise to an augmentation of metabolic disturbance including hypercholesterolemia 2.1. Alga Material and may lead health problems such as diabetes mellitus, steatosis and atherosclerosis Experimental procedure and Extraction of alga material. 2005; Hassan et al, 2011). C. gracilis was collected from Sfax coast, Tunisia, high level of serum TC (total cholesterol), small density in April 2016. This macroalga was authenticated at in lipoprotein cholesterol and a high level density Botany department, Faculty of Science Sfax, Tunisia

Results

Discussion

Conclusion