Effects of Cudrania tricuspidata Leaf on Serum Lipids and Antioxidant Enzyme Activity in Rats on High-Fat Diets

Abstract

꾸지뽕잎이 고지방식이를 섭취한 성장기 수컷 쥐에서 혈중 지질 농도와 간 지질 함량 및 항산화 효소활성에 미치는 영향을 아래와 같이 요약하였다. 평균 식이섭취량은 고지방군이 대조군과 고지방+꾸지뽕군에 비하여 유의적으로 낮았고(p<0.05), 체중증가량도 유의적으로 낮았다(p<0.05). 식이효율은 고지방군, 고지방+꾸지뽕군이 대조군에 비하여 유의적으로 높았다(p<0.05). 혈중 총 콜레스테롤, LDL-콜레스테롤은 고지방+꾸지뽕군이 고지방군에 비하여 유의적으로 낮았다(p<0.05). 혈중 중성지방, HDL-콜레스테롤, 동맥경화지수는 고지방, 꾸지뽕 섭취에 따른 유의적인 차이가 없었다. 간의 총 콜레스테롤과 중성지방 함량은 고지방, 꾸지뽕 섭취에 따른 유의적인 차이가 없었다. 간 조직의 MDA 함량은 고지방군과 고지방+꾸지뽕군이 대조군에 비하여 유의적으로 높았다(p<0.05). 간 조직의 SOD 활성은 고지방+꾸지뽕군이 대조군과 고지방군에 비하여 유의적으로 높았으며(p<0.05), GSH-Px 활성, CAT 활성은 고지방, 꾸지뽕 섭취에 따른 유의적인 차이가 없었다. 결론적으로 고지방을 유도한 성장기 쥐에게 꾸지뽕잎 10%(100 g/kg dry diet) 첨가식이를 섭취시켰을 때 혈중 총 콜레스테롤과LDL-콜레스테롤은 유의적으로 낮았고, 간 조직의 SOD 활성은 유의적으로 높게 나타났다. 따라서 꾸지뽕잎은고지방을 섭취한 성장기 수컷 쥐에게 혈중 콜레스테롤 및 간 조직의 SOD 활성에 개선 효과를 보였다.The purpose of this study was to investigate the effects of Cudrania tricuspidata on serum lipids and antioxidant enzymes in growing rats fed a high-fat lard diet for six weeks. Twenty-four male Sprague-Dawley rats (body weight 50-60 g) were divided into three groups: control, high-fat diet (lard at 200 g/kg), and high-fat diet + Cudrania tricuspidata (lard 200 g/kg + Cudrania tricuspidata 100 g/kg). The results of the study indicate that mean intake was significantly lower for subjects in the high-fat diet group than for those in the control group and the high-fat + Cudrania tricuspidata group. Body weight gain was significantly lower in the high-fat diet group than in the control group and the high-fat diet + Cudrania tricuspidata group. The food efficiency ratio was significantly higher in the high-fat diet and high-fat + Cudrania tricuspidata groups than in the control group. The levels of serum total cholesterol and LDL cholesterol were significantly lower in the high-fat diet + Cudrania tricuspidata group than in the high-fat diet group. The levels of serum triglycerides and HDL cholesterol, and the atherogenic index (AI) did not significantly differ among the groups. The levels of liver total cholesterol and triglycerides did not significantly differ between the high-fat diet and the Cudrania tricuspidata-supplemented diet. The concentration of lipid peroxidation by measuring malondialdehyde (MDA) was significantly higher in high-fat diet group and in the Cudrania tricuspidata group than in the control group. Antioxidant enzymes in liver tissue, such as superoxide dismutase (SOD), were significantly higher in the high-fat diet + Cudrania tricuspidata group than in the control group and the high-fat diet group. Glutathione peroxidase (GSH-Px) and catalase (CAT) did not significantly differ between the lard-only diet and the Cudrania tricuspidata-supplemented diet. In conclusion, it is possible that Cudrania tricuspidata-supplemented diets may produce positive results on serum total cholesterol, LDL cholesterol, and antioxidant enzymes (such as SOD) in liver tissue.