Abstract

Abstract. There are many new optical polarimetric methods of biological tissue structure assessment, which ones are the most perspective in PMI estimation. The purpose of the work is to develop and to test the method of two-dimensional Stokes-polarimetric mapping of biological layers autofluorescence for PMI estimation by statistical analysis of postmortem changes dynamics of the coordinate distributions values of polycrystalline films of liquor (PFL) images laser-induced fluorescence polarization azimuth (LIFPA). Materials and methods. Objects of investigation are PFL, taken in 78 corpses with accurately known time of death and 20 healthy volunteers. Coordinate distributions of LIFPA image values were determined for each sample of PFL in the optical arrangement of the Stokes polarimeter in short-wave spectral bands of optical emission. Results and conclusions. Two-dimensional Stokes-polarimetric mapping distributions LIFPA of PFL images may be used in PMI estimation. Statistical points of the 3 rd and 4 th orders are the most sensitive PFL optical values to evaluate postmortem changes by short-range fluorescence. Optimal interval in 24 hours and the accuracy of PMI estimation 25 min for proteins and NADH were found.

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