Abstract
The renaturation following rapid cooling of heat-denatured DNA extracted from mitomycin-treated Escherichia coli is demonstrated. When mitomycin-treated cells of E. coli strain B are incubated, following treatment in a growing medium, but not in a non-growing medium, it is no longer renaturable after heat denaturation. This is interpreted to mean that the interstrand cross-links in DNA caused by mitomycin are broken during post-treatment incubation. The cross-linked DNA of mitomycin-treated mutants of E. coli, lacking host-cell or dark reactivating enzymes is not modified by post-treatment incubation. Sensitivity to mitomycin is to some extent, but not entirely, correlated with the absence of host-cell reactivating enzymes and their effect on cross-linked DNA.
Published Version
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