Possible role of isocitrate dehydrogenase gene mutation in the genesis of glioma

Abstract

Objective To explore the possible role of isocitrate dehydrogenase (IDH) gene mutation in the genesis of glioma. Methods DNA was extracted from 89 glioma FFPE specimens. The primers were designed and synthetized. The exon 4 of IDH1 and IDH2 gene was screened and analyzed through polymerase chain reaction (PCR) and direct DNA sequencing. Results Altogether 36 IDH1 gene mutations were found (40. 4% ) , mainly from WHO Ⅱ and Ⅲ grade astrocytoma (61%-63% ) , oligodondroglioma (33%-66%) and oligoastrocytoma (57% -80%). All mutations were heterozygous, missense and point mutation, which were only found in codon 132 of IDH1. The patients with gene mutation were younger than those without muatation. Conclusion IDH1 gene mutation may play an important role in the tumorigenesis of glioma. Key words: IDH; Gene mutation; Glioma