Abstract
The objective of the study was to develop and evaluate a portable device for continuous fractionated blood sampling and continuous ex vivo monitoring of blood glucose. The inner lumen of a double lumen catheter (18 gauge × 45 mm) was placed in a peripheral vein and perfused with heparin solution (1·4 U min −1). The outer lumen was used to collect heparinized blood into 48 vacuum tubes at programmable sample volumes and time intervals (0·2-2 ml in 2·5–30 min). A sensor flow chamber with an internal volume of 1 mm 3 incorporating a miniaturized thin-film amperometric glucose sensor was placed in the sampling line for continuous ex vivo blood glucose monitoring. Blood glucose and plasma insulin were measured during a frequently sampled intravenous glucose tolerance test (250 mg kg −1) and a subsequent oral glucose tolerance test (150 g) over 6 h in eight healthy volunteers (BMI 24·5 ± 3·2 kg m −2). Additionally, in four experiments blood glucose was measured on-line using the glucose sensors. The overall correlation coefficients for whole blood glucose and plasma insulin between the manually drawn samples and the vacuum tubes were 0·73 and 0·87, respectively ( p < 0·001). The miniaturized glucose sensor exhibited a linear measuring range of 25 mmol −1 glucose concentration and 95% response times of less than 30 s. Sensor readings and laboratory analyser results for the blood glucose measurement correlated between 0·93 and 0·98 ( p < 0·001). In summary, continuous fractionated blood sampling and ex vivo blood glucose monitoring in ambulatory subjects is possible with a portable device.
Published Version
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