Abstract
AbstractBone regeneration can be accelerated by localized delivery of appropriate growth factors/bio active molecules. Localized delivery can be achieved by incorporating bioactive molecules within biodegradable particulate carrier system followed by embed them in a suitable porous scaffolds. These carrier system facilitates the impregnated growth factor(s) to release at a desirable rate and concentration, and to linger at injury sites for a sufficient time to recruit progenitors and stimulate tissue healing processes. In this study, an attempt has been made to engraft the porous chitosan-gelatin scaffolds with PLGA nanoparticles for localized delivery of bioactive components. Scaffolds loaded with PLGA nanoparticles were subjected to physical and mechanical characterizations such as microarchitecture analysis, swelling, porosity, mechanical properties, dissolution studies.
Highlights
In the domain of tissue engineering, the repair of skeletal defects is an immediate clinical need as impaired fracture healing or nonunion bone defects often result in a functional disability for which there is no effective therapy [1].Conventional tissue engineering strategies utilize combination of biodegradable porous scaffolds and bioactive molecules such as growth factors to mimic natural processes of healing [2]
The aim of this work is to prepare chitosan-gelatin (CH/G) scaffolds embedded with PLGA nanoparticles for the purpose of localized growth factor delivery at a pre-determined rate and to improve the therapeutic efficiency of scaffolds by mimicking the endogenous release of bioactive substance(s)
PLGA nanoparticles formed ere spherical in shape and displayed a smooth surface with an average size below 200 nm (Fig.4)
Summary
In the domain of tissue engineering, the repair of skeletal defects is an immediate clinical need as impaired fracture healing or nonunion bone defects often result in a functional disability for which there is no effective therapy [1].Conventional tissue engineering strategies utilize combination of biodegradable porous scaffolds and bioactive molecules such as growth factors to mimic natural processes of healing [2]. Such approaches often produce unpredictable results, probably due to the short biological half life of bioactive molecules and lack of long term stability and tissue specificity [3]. The aim of this work is to prepare chitosan-gelatin (CH/G) scaffolds embedded with PLGA nanoparticles for the purpose of localized growth factor delivery at a pre-determined rate and to improve the therapeutic efficiency of scaffolds by mimicking the endogenous release of bioactive substance(s)
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