Abstract
Simple SummaryIncreasing milk fat rate has a good effect on the milk quality of cattleyak. SNPs can help us find potential molecular markers for the milk fat traits of cattleyak, and they can be screened according to molecular markers when they are young. It provides a reference for cultivating high milk fat cattle population in the future. The results of this study suggest that the SORBS1 gene polymorphism is closely related to the milk fat traits of cattleyak, which could be used as a candidate genetic marker for milk fat trait selection in cattleyak. This study provides a new molecular marker and theoretical basis for screening the milk fat traits of cattleyak. It has a certain reference value for the research and improvement of milk quality.This study aimed to find the SNPs in the SORBS1 gene of cattleyak, analyze the relationship between its polymorphisms and the milk fat traits, and find potential molecular markers for the milk fat traits of cattleyak. The polymorphism of the SORBS1 gene in 350 cattleyak from Hongyuan County (Sichuan, China) were detected by PCR and DNA sequencing, and the correlation between these SNPs and the milk production traits of cattleyak was analyzed. The results showed that there were nine SNPs in the CDS and their adjacent non-coding regions of the SORBS1 gene, and all SNPs have three genotypes. The correlation analysis found that the genotypes with superior milk fat traits in the other eight alleles were homozygous genotypes with a high genotype frequency except the g.96284 G > A (c.3090 G > A) (p < 0.05). However, at locus g.96284 G > A, the milk fat percentage, monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs) and saturated fatty acids (SFAs) of the GA genotype were significantly higher than that of GG and AA genotypes (p < 0.05). Among these SNPs, three SNPs (g.6256 C > T (c.298 C > T), g.24791 A > G (c.706 A > G) and g.29121 A > G (c.979 A > G)) caused the amino acids change. The genotypes of the three SNPs consist of three haplotypes and four diplotypes. The amino acid mutation degree of diplotype H1–H1 (CCAAAA) was the highest, and its milk fat percentage, MUFAs, PUFAs and SFAs were also the highest (p < 0.05). Taken together, we found nine SNPs in the SORBS1 gene that are closely related to the milk fat traits of cattleyak. Moreover, the mutation of amino acids caused by SNPs had positive effects on the milk fat traits of cattleyak. H1-H1 is the dominant diplotype which significantly related to the milk fat traits of cattleyak. This study provides a new molecular marker and theoretical basis for screening the milk fat traits of cattleyak.
Highlights
Yak (Bos grunniens), a wild bovine species that lives 3500 m above sea level, is an iconic symbol of the Qinghai–Tibetan Plateau region and the nearby areas
The cattleyak is the subject of this experiment, the detected SNPs of the sorbin and SH3 domain-containing 1 (SORBS1) gene and its association with cattleyak milk fat traits was analyzed, and we looked for the molecular markers for the assisted selection of the milk fat traits of cattleyak
G > A) are located in the CDS region, while the other five SNPs are located in the intron region adjacent of the SORBS1 gene
Summary
Yak (Bos grunniens), a wild bovine species that lives 3500 m above sea level, is an iconic symbol of the Qinghai–Tibetan Plateau region and the nearby areas. Yak raising has become the main livelihood in the local area. The reproductive efficiency and production performance of yak are low, which cannot satisfy the local herdsman needs and industrial development [1]. As the hybrid offspring of yak (♀) and cattle (Bos taurus) (♂), the female cattleyak has obvious heterosis compared to yak, especially in its lactation performance [2,3]. Dairy products made from fermented and processed milk are popular with local herdsmen and tourists. As the main energy substance in milk, milk fat only accounts for 3~5% of the milk content, but it determines the nutritional value of milk [4,5]. It is greatly meaningful to increase the percentage of milk fat and improve the composition of fatty acids in cattleyak
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
