Abstract
The aim of present study was to identify the polymorphisms in genes encodingcalpastatin (CASTUoG), calpain (CAPN1, CAPN2), diacylglycerol Oacyltransferase(DGAT1), thyroglobulin (TG5), and Stearoyl-CoA Desaturase(SCD) in order to analyse genetic structure of Pinzgau cattle. The genomic DNAfor genotyping was obtained from in total 56 blood samples of Pinzgau bulls. Afterextraction, the concentration of DNA was controlled by the spectrophotometrymeasurement. The genotyping of each individual was carried out by using PCRRFLPmethods. The average value of observed (0.37±0.05) and expectedheterozygosity (0.39±0.06) clearly indicated the prevalence of homozygousindividuals. Observed Wright's fixation indexes showed positive values across allloci (0.03±0.06), which confirmed slight deficiency of heterozygote animalscompared to the Hardy-Weinberg equilibrium expectations. The Hardy-Weinbergequilibrium was found in population, which signalizes only slight impact of factorssuch as selection, migration or inbreeding. The effectiveness of loci allele impact inpopulations has been described also by effective allele numbers (1.68±0.13) thatexpressed the decrease of allele activity in population. The loss of heterozygosityin analysed population was found across all of genetic markers. Each of theevaluated indicators clearly points to the need of genetic diversity monitoring.Moreover, the analyses of single nucleotide polymorphisms in genes significantlyinvolved in control of economically important production traits are still very usablemethods for identification of genetic markers that can be used in marker assistedselection of cattle.
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