Polymorphism in the apolipoprotein C3 gene and coronary lesions in patients with CHD

Abstract

Background and Aims : The aim of our study was to evaluate the distribution of the APOC3 SstI polymorphism and its impact on the character of dyslipidemia and features of coronary lesions in Uzbek patients with unstable angina.Methods: The study included 141 patients with unstable angina pectoris (HC) class IIB (Braunwald E. et al., 1989) with LDL cholesterol> 100 mg / dL. The comparison group consisted of 50 healthy, age-matched, randomly selected individuals without clinical and instrumental signs of coronary artery disease according to the results of an exercise test. Coronary angiography was performed on an Allura CV-20 (Philips, Netherlands). Genotyping of SstI APOC3 gene polymorphism by PCR-RFLP. Alleles lacking a restriction site were designated S1, and alleles containing an SstI site were designated S2.Results: The analysis of the APOC3 SstI polymorphism revealed a significant prevalence of carriers of the S2 allele among patients with UA compared with healthy ethnic Uzbeks. Carriage of the S2 allele was associated with hypertriglyceridemia (> 230 mg / dL) (OR = 2.30, 95% CI: 1.14-4.67; χ2 = 5.485; P = 0.02) and a higher risk of three- and multivessel lesions. (OR = 2.171, 95% CI: 1.0792-4.3680; P = 0.03).Conclusions: Our results indicate that the determination of APOC3 SstI polymorphism may be a useful additional marker in assessing cardiovascular risk and indications for coronary angiography. Background and Aims : The aim of our study was to evaluate the distribution of the APOC3 SstI polymorphism and its impact on the character of dyslipidemia and features of coronary lesions in Uzbek patients with unstable angina. Methods: The study included 141 patients with unstable angina pectoris (HC) class IIB (Braunwald E. et al., 1989) with LDL cholesterol> 100 mg / dL. The comparison group consisted of 50 healthy, age-matched, randomly selected individuals without clinical and instrumental signs of coronary artery disease according to the results of an exercise test. Coronary angiography was performed on an Allura CV-20 (Philips, Netherlands). Genotyping of SstI APOC3 gene polymorphism by PCR-RFLP. Alleles lacking a restriction site were designated S1, and alleles containing an SstI site were designated S2. Results: The analysis of the APOC3 SstI polymorphism revealed a significant prevalence of carriers of the S2 allele among patients with UA compared with healthy ethnic Uzbeks. Carriage of the S2 allele was associated with hypertriglyceridemia (> 230 mg / dL) (OR = 2.30, 95% CI: 1.14-4.67; χ2 = 5.485; P = 0.02) and a higher risk of three- and multivessel lesions. (OR = 2.171, 95% CI: 1.0792-4.3680; P = 0.03). Conclusions: Our results indicate that the determination of APOC3 SstI polymorphism may be a useful additional marker in assessing cardiovascular risk and indications for coronary angiography.