Abstract
O estabelecimento de cultura in vitro de Polygala paniculata L. permitiu a propagação rápida de matéria-prima vegetal sob condições assépticas e padronizadas. As plantas in vitro foram submetidas à extração e destilação simultâneas (EDS) e a composição química do óleo essencial foi analisada por CG/DIC e CG/EM. Foram avaliados os efeitos de BAP (6-benzilaminopurina) sobre o desenvolvimento in vitro e produção de voláteis em P. paniculata. A principal característica do óleo essencial consistiu na presença marcante de salicilato de metila. Plantas cultivadas em meio controle MS (Murashige & Skoog) produziram mais do que 80% de salicilato, enquanto o uso de BAP, reduziu a produção de salicilato de metila a 71% e 21% para as concentrações de 2 e 4 mg L-1, respectivamente. A cultura de tecidos de P. paniculata pode ser indicada como fonte de salicilato de metila.
Highlights
Methyl salicylate known as salicylic acid methyl ester is used in flavoring for foods, candies, beverages and pharmaceuticals
The main volatile compound detected in P. paniculata root was methyl salicylate (Nogueira et al, 2005)
This study indicated a high methyl salicylate production in leaves and stems
Summary
Methyl salicylate known as salicylic acid methyl ester is used in flavoring for foods, candies, beverages and pharmaceuticals. It is an odorant, perfume and ultraviolet absorber in cosmetics. This compound is used in traditional medicine, mainly as anti-inflammatory, analgesic, expectorant and anti-rheumatics (Effmert et al, 2005). The main volatile compound detected in P. paniculata root was methyl salicylate (Nogueira et al, 2005). This volatile is the main component of Gelol®, a common medicine in Brazil. Previous studies have shown scientific evidence for its analgesic, antiedematogenic and gastroprotective activities (Nogueira et al, 2005; Lapa et al, 2007; Lorenzi & Matos, 2008)
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