Abstract
The polarographic behaviour at the DME of vitamin B12 has been studied in pure aqueous, water-methanol, water-ethanol and water-isopropanol solutions, in presence of 0.1 M LiCl as indifferent electrolyte. In pure aqueous solutions two waves are observed. The first one is a two-electron step and the second wave is a catalytic hydrogen wave. In water-methanol solutions the polarograms contain the two normal waves. In water-ethanol solutions containing more than 70 vol.% alcohol and in water-isopropanol solutions containing more than 40 vol.% alcohol, the first wave of vitamin B12 splits into two waves. The effect of the composition of the solvent on id, on E1/2 and on the kinetics of the electrode process have been investigated. The reduction mechanism of vitamin B12 and the nature of the waves are discussed.
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