Abstract
The interaction of Cu2+, Mn2+ and Ca2+ ions with DNA in aqueous and water-ethanol solutions at different metal ion concentrations was studied by IR-spectroscopy. At sufficiently high DNA concentrations, DNA interaction with Cu2+, Ca2+ and Mn2+ ions results in compacting of DNA in the aqueous solutions. This process shows a very high cooperativity. In the presence of alcohol, DNA condensation takes place at much lower concentrations of metal ions used than in pure aqueous solution. Binding constants and cooperativity of the metal ion binding rise, and the non-monotonous dependencies of the binding degree, r, on the concentration of free metal ions, Cf, become pronounced. Binding isotherms take the S-like form similar to van der Waals isotherms for phase transitions of the liquid-vapour type. Cu2+ and Ca2+ ion binding to DNA in water-ethanol solutions also results in compacting of DNA macromolecule. The process is characterised by a high positive cooperativity and has a phase transition character.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
