PHYTOCHEMICAL SCREENING AND STUDY OF ANTI-MICROBIAL, ANTI-HAEMOLYTIC, ANTI-FUNGAL AND ANTI-OXIDANT ACTIVITIES OF ETHANOL EXTRACT OF PANICUM SUMATRENSE LEAVES

Abstract

Little millets (Panicum Sumatrense) are of immense importance both medically and economically. Scientifically proven that the grains of little millets are nutritionally well. In the present research work, the less explored, underutilized, nutrient-rich and easily available Panicum sumatrense leaves were analysed for their phytochemical constituents and anti-oxidant or anti-haemolytic activity. The ethanolic extract of the little millet was examined to proximate, phytochemical determinations and anti-oxidant, anti–haemolytic, anti-bacterial and anti-fungal activity. It was found that the given sample contains important Phytochemicals. The extract prepared using leaves of Panicum sumatrense was evaluated for their antioxidant activity, anti-haemolytic activity using UV-Vis spectrophotometer. The antibacterial and antifungal activity tests were performed using the Agar well diffusion method. Anti-bacterial activity of ethyl alcohol extract and n-hexane extract was higher for Escherichia coli and In Staphylococcus aureus extract was slightly low effective. Anti-fungal activity of ethanolic and n-hexane extract was not satisfactory. The leaves extract showed that both anti-oxidant, anti-hemolytic and anti-bacterial activity increased with increasing concentration. Thus, the result revealed the anti-oxidant anti-haemolytic and anti-bacterial activity of the underutilized leaves of Panicum sumatrense.