Abstract
This Research on the antibacterial and antifungal activity of nanosilver against Neisseria gonorrhoeae and Candida albincas fungi has been carried out. The purpose of this study was to determine antibacterial activity of nanosilver against Neisseria gonorrhoeae and antifungal activity against Candida albincas. Synthesis Nanosilver uses bottom up method and characterized using UV-Vis Spectrophotometer. Nanosliver concentrations used were 30, 40, 50, and 60 ppm. Antibacterial and antifungal activity tests using disk diffusion method. Observations obtained in form of the presence or absence of clear zones formed around paper discs indicate the inhibition of nanosilver on microbial growth. The results of testing the antifungal activity of Candida albicans on nanosilver with concentrations of 30, 40, 50 and 60 ppm resulted in clear zones of 9.73 nm, 11.46 nm, 11.93 nm, and 13 nm with fungal inhibition response categories is medium and strong. The results antibacterial activity test of Neisseria gonorrhoeae on nanosilver with concentrations of 30, 40, 50 and 60 ppm did not show any clear zone around the disc, it showed that nanosilver in this study did not have antibacterial activity against Neisseria gonorrhoeae.
Highlights
In this era of globalization there have been many advances in all fields and aspects of science about the importance of hygiene and the basic health of physical and organ hygiene
This research was carried out using several materials including 250 ml beaker glass, 10 ml beaker glass, 50 ml beaker glass, hot plate and stirrer, Ohauss analytical balance, spatula, 100 ml measuring flask, Shimadzu UV-Vis Spectrophotometer 1800, micropipette, autoclave, incubator, calipers, ose needles, tweezers, vortex, petri dishes, AgNO3 (≥99%, Merck), sodium citrate (99%, Merck), aquades (PT Bratachem), aquabides (PT Bratachem), liquid media Potato Dextrosa Agar (DPA), ketocenazole, dimethyl sufoxide (DMSO), Neisseria gonnorhoeae stock colony, Candida albicans stock colony, filter paper, paper discs, Ciprofloxacin
1000 ppm AgNO3 solution was obtained by dissolving 1.57 g of AgNO3 crystal which was put into a 1000 ml volumetric flask, diluted with aquabides to mark limits. the mixture is homogeneous and a 1000 ppm AgNO3 solution is formed which is ready to be used as material in nanosilver synthesis. 1000 ppm AgNO3 is used for synthesis
Summary
In this era of globalization there have been many advances in all fields and aspects of science about the importance of hygiene and the basic health of physical and organ hygiene. One of the organs that really needs special care is the reproductive organs. Reproductive organs are one of the important things in every human life. Reproductive organs are the subject of several diseases. Knowledge and understanding of the health of reproductive organs can be used as prevention for men and women so that they will be better able to maintain the health of their reproductive organs.[1]. The vaginal canal is very vulnerable to risk of infection from the outside. The wrong way to clean the vagina and leave the condition of the vagina moist triggers the disease in female reproductive organs [2]. There is a term called Vaginal Infection. Vaginal infection is one of the most frequent problems where women visit a doctor [3].
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