Abstract
The marine ecology is diverse with innumerable types of natural substances, both of plant and animal origin. Padina tetrastromatica (PT) (Hauck) is a brown algae belonging to the order Dictyotales, found in coastal region. The objective of present investigation was to evaluate phytochemical profile of extracts of PT. The air dried plant material was defatted and extracted successively with solvents of increasing polarity. Incumbent study was performed with standard qualitative phytochemical tests and HPTLC fingerprint analysis using CAMAG HPTLC system. The results showed the presence of phytoconstituents like sterols, terpenoids, flavonoids, glycosides, alkaloids and carbohydrates. Furthermore, components present in extracts were resolved in best possible solvent system by HPTLC. The chloroform extract of PT displayed eight peaks, in which those with Rf values 0.28 and 0.72 were more predominant. Whereas ethanol extract of PT exhibited nine peaks, in which maximum Rf value was found to be 0.82. In conclusion, the data of this study provide useful guide and suitability for investigation of biological activity of the plant according to the phytochemical groups observed. However, further work is needed to standardize the above chemical constituents in comparison with biomarker and this result can also be measured along with the other data for setting up the standards to this plant.
Highlights
Since decades, herbs or plants are used as an imperative resource of medicines due to the presence of bioactive components having therapeutic and pharmaceutical significance [1]
High performance thin layer chromatography (HPTLC) finger print analysis can serve as potent tool for identification, authentication and quality control of herbal medicine
Preliminary phytochemical study of extracts The preliminary phytochemical study of extracts of Padina tetrastromatica (PT) was performed to reveal the presence of different secondary metabolites
Summary
Herbs or plants are used as an imperative resource of medicines due to the presence of bioactive components having therapeutic and pharmaceutical significance [1]. Standardization of these plant materials is essential days, which can be achieved by use of current methods available for describing the identification and quantification of active constituents [2]. High performance thin layer chromatography (HPTLC) finger print analysis can serve as potent tool for identification, authentication and quality control of herbal medicine This technique could be considered as a good alternative, because of its simplicity and reliability [3].
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