Abstract
The present study embraces phytochemical investigation of the essential oil extracted from the mature seeds of Trachyspermum ammi Linn for different constituents by subjecting the oil to gas chromatography-mass spectrometry (GC-MS) analysis. The identification of the constituents is based upon retention indices and by comparison of their mass spectral fragmentation patterns against the commercial library mass spectra (Wiley, Nist etc.). Ellagic acid (EA), which is a natural phenol antioxidant, has been isolated from methanol extract from the mature seeds of Trachyspermum ammi Linn. Also, Thymol (Thl), a naturally occurring phenolic compound, has been crystallized by the reported standard procedure from oil extracted from these mature seeds. Both these compounds have been evaluated for their possible anti-cancer effect against a selected panel of human cancer cell lines by means of sulforhodamine B assay.
Highlights
Environmental Sciences and its ApplicationsTrachyspermum ammi Linn Synonym Carum copticum Linn (Family: Umbelliferae or Apiaceae) is widely recognized as ajowan or ajwain in India.Vol 3, No 2 March 2017 pp. 91–100Cultivation of this plant species was instigated in Egypt
3.1 gas chromatography-mass spectrometry (GC-MS) analysis of the oil extracted from mature seeds of Trachyspermum ammi Linn
The present work reveals analysis of the essential oil extracted from mature seeds of Trachyspermum ammi Linn for different constituents by gas chromatography-mass spectrometry (GC-MS) analysis
Summary
Environmental Sciences and its ApplicationsTrachyspermum ammi Linn Synonym Carum copticum Linn (Family: Umbelliferae or Apiaceae) is widely recognized as ajowan or ajwain in India.Vol 3, No 2 March 2017 pp. 91–100Cultivation of this plant species was instigated in Egypt. Ajwain has been extensively employed in the traditional ayurvedic and unani medicines for different ailments and as enhancer of body’s resistance Asif et al (2014). Being motivated by the extensive pharmacological activities and medicinal applications of Trachyspermum ammi Linn, we have analyzed the essential oil extracted from mature seeds for different constituents by subjecting the oil to hyphenated GC-MS technique. Thymol has been crystallized by the reported standard procedure of storing the oil extracted from mature seeds under low temperature condition for overnight Guenther (1950) These isolated compounds have been evaluated for their possible anticancer potential against a panel of selected human cancer cell lines by Sulforhodamine B assay Monks et al (1991)
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