Abstract
Inflorescence of Musa species is one of the most commonly consumed vegetables in Southeast Asian region. In the present study, chemical composition and antioxidant potential of the ethanolic extract of inflorescence of Musa balbisiana Colla (MbCi) were evaluated. In addition, the extract was also subjected to cytotoxicity testing on a panel of human cancer cell lines. The ethanolic extract of inflorescence of Musa balbisiana Colla was evaluated for antioxidant activity using 1,1-diphenyl-2-picryl-hydrazyl assay (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) assays. The extract was chemically characterized for total phenolic (TPC) and total flavonoid (TFC) contents and by gas chromatographymass spectrometry (GC-MS) analysis. Cytotoxicity was evaluated by MTT cell viability assay. The extract showed moderate antioxidant activity in all the antioxidant assays. Chemically, the extract was found to possess high total phenolic (92.01 ± 0.40 μg gallic acid equiv./mg extract) but low flavonoid (4.852 ± 0.04 μg quercetin equiv./mg extract) contents. In cell viability assay, MbCi extract showed selective cytotoxicity towards HT-29 cell line. Morphological observations show that MbCi has apoptosis inducing nature. GC-MS analysis has revealed the presence of 22 compounds, mainly belonging to steroids, fatty acids and long chain aliphatic compounds, which in part may be responsible for observed antioxidant and cytotoxic activities of ethanol extract. Our study revealed that MbCi has chemotherapeutic potential activity that warrants further investigation.
Highlights
Plants from genus Musa contribute as the fourth most important food in the world today
The ethanolic extract of inflorescence of Musa balbisiana Colla was evaluated for antioxidant activity using 1,1-diphenyl-2-picryl-hydrazyl assay (DPPH), 2,2'-azino-bis (3ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) assays
The extract was chemically characterized for total phenolic (TPC) and total flavonoid (TFC) contents and by gas chromatography–mass spectrometry (GC-MS) analysis
Summary
Plants from genus Musa contribute as the fourth most important food in the world today. Inflorescence of Musa species (banana) is much appreciated vegetable in Malaysia. Decoction of half ripe fruit of Musa balbisiana Colla (MbC) is used in the Revadigar et al / Journal of Applied Pharmaceutical Science 7 (05); 2017: 103-110. Fresh dried pulp of the fruit was reported to possess in vitro as well in vivo antioxidant activity in a dose dependent manner (Mudoi et al, 2011). Antioxidant activity of eight Malaysian bananas were reported by Sulaiman et al (2011), who found that the chloroform extract of dried pulp possessed good antioxidant activity. Vilela et al (2014) identified GC-MS of dichloromethane extracts of several Musa species and reported the presence of lipophilic phytochemicals. There is no data available on the antioxidant activity and cytotoxicity of inflorescence of Musa balbisiana Colla (MbCi). The present study was designed to investigate the phytochemical composition, antioxidant capacity and cytotoxicity of MbCi
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