Abstract
Roselle (Hibiscus sabdariffa L) belongs to the Malvaceae family is a multipurpose plant with high economic importance and worldwide fame for its nutritional and therapeutic value, but unfortunately, it is underutilized in India. Their calyces are utilized as ethnic food and in traditional medicine to cure many degenerative diseases in different parts of the world and by the tribal of India. However, no evidence is available in the literature regarding the existent quality assessment of the roselle calyces in India. Therefore, the objective of the study was to characterize the calyces of roselle (Hibiscus sabdariffa L.) from India. The calyces were grounded, sieved to 20 mesh sizes, and stored in the refrigerator for further analysis. The parameters analyzed included nutritional composition and the biologically effective component that is total phenols and the total anthocyanins. A procedure to measure the anthocyanins by pH-differential UV-visible spectrometry was followed. Total phenolic content was assessed by the Folin Ciocalteau method, and gallic acid was used as a standard. The individual anthocyanins present in Hibiscus were characterized by TLC. The results reveal that these indigenous powdered calyces are rich sources of macro and micronutrients, bioactive components like polyphenols and flavonoids, especially anthocyanins. Our study substantiates the health /nutritional claims and validates its applicability in functional food processing and therapeutic treatments.
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