Physiological Response to Low Temperature of Four Genotypes of Cyclocarya paliurus and Their Preliminary Evaluation to Cold Resistance

Abstract

Cyclocarya paliurus is a versatile tree species with immense potential for development, as it combines edible, medicinal, and ornamental functions. Low temperature is one of the important abiotic factors that affect plant survival and flourishing but their response mechanism to low temperature is not yet clear. In this study, we utilized annual shoots of four genotypes of C. paliurus, namely T2, W10, M31 and S12, as materials. The physiological responses of annual shoots of C. paliurus to low temperature stress were elaborated by determining and comparing indicators related to cold resistance, such as relative electric conductivity, semi-lethal low temperature, malondialdehyde, soluble sugar, soluble protein, proline, superoxide dismutase and peroxidase. The contents of malondialdehyde, proline, soluble protein and peroxidase activities were not only correlated with the treatment temperature but also related to the genotypes. Osmotic substance (soluble sugar, soluble protein and proline) contents and antioxidant enzyme activities (peroxidase and superoxide dismutase) of the four genotypes showed a trend of increasing and then decreasing with the five decreasing temperatures. Furthermore, a comprehensive evaluation of cold resistance was performed by using a combination of principal component analysis and membership function, with the cold resistance ranked as W10 > M31 > S12 > T2. Results from this study would provide some references for extending the plantation areas.