Abstract
Background: Thyroid-stimulating hormone (TSH) is a member of the vertebrate glycoprotein hormone family [1]. It’s secreted from pituitary cells as heterodimers composed of an alpha and a beta- subunit. The thyrotrophic cells that secrete TSH are preferentially located in the anteromedial and anterolateral portions of the pituitary. Objective: We performed a phylogenetic analysis of the TSH, and phylogram of the maximum likelihood relations between TSH coding sequences of five representative species. Methods: We extracted the available DNA and protein sequences for TSH from the NCBI databank. Searched for regions presenting sequence similarities to the constituent domains of TSH – alpha and beta-subunits - with the Blastall command ftp://ftp.ncbi.nlm.nih.gov/blast/db/ website, pairs of sequences were compared on the basis of their global alignment with the Myers & Millers algorithm manpages.ubuntu. com/manpages/karmic/man1, and the phylogenetic reconstructions were performed online by using a maximum likelihood method with PhyMyL 3.0 software on the website file (http://www.phylogeny.fr/). Results: The comparison of the phylogenetic trees that we obtained compared with those studies previously published revealed similar subclusters with high protein homology. Conclusion: This study demonstrates that human TSH is structurally related to TSH of the species analysed, Bos taurus, Mus musculus, Canis lupus familiaris, and Cyprinus carpio, respectively.
Highlights
Thyroid-stimulating hormone (TSH) is a member of the vertebrate glycoprotein hormone family.(1) It is a heterodimeric 28-kDa-glycoprotein hormone released from the anteromedial and anterolateral portions of the pituitary gland and is a regulator of thyroid function
We performed a phylogenetic analysis of the TSH (Figure 3), and phylogram of the maximum likelihood relationships between TSH coding sequences of five representative species
The human TSH beta-subunit gene structure had 4.5 kilobases (Kb) in size and it was separated into three exons
Summary
Thyroid-stimulating hormone (TSH) is a member of the vertebrate glycoprotein hormone family.(1) It is a heterodimeric 28-kDa-glycoprotein hormone released from the anteromedial and anterolateral portions of the pituitary gland and is a regulator of thyroid function. The human TSH protein structure has been studied in detail (Figure 1). It’s secreted from pituitary cells as heterodimers composed of an alpha and a beta- subunit. Objective: We performed a phylogenetic analysis of the TSH, and phylogram of the maximum likelihood relations between TSH coding sequences of five representative species. Searched for regions presenting sequence similarities to the constituent domains of TSH – alpha and beta-subunits - with the Blastall command ftp://ftp.ncbi.nlm.nih.gov/blast/db/ website, pairs of sequences were compared on the basis of their global alignment with the Myers & Millers algorithm manpages.ubuntu.
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