Abstract
Raman, fluorescence and FTIR experiments of prestine Sagittula stellata and Sagittula stellata–metal ion complexes grown in light and in dark were performed to probe the photosensitivity response of the cellular components in the marine bacterium. In the presence of Cu(ii) and Zn(ii) the frequency shifts of PO2−, C–O–C and C–O–P vibrations indicate metal binding to nucleic acids, carbohydrates and polysaccharides. We assign the observed bands in the 514.1 nm Raman spectra of the prestine S. Stellata and of the extracted carotenoids to the C Created by potrace 1.16, written by Peter Selinger 2001-2019 ]]> C and C–C stretching vibrations. The fluorescence excitation–emission matrix (EEM) of S. stellata in light, dark and in the presence of metal ions are reported and compared with the Raman and FTIR data. The novel ability of S. stellata although heterotrophic, to show light-dependent metal binding ability may be an important feature property that maintains a stable heterotroph–prototroph interaction and a dynamic system.
Highlights
Marine Roseobacter clade bacteria (RCB) are one of the most abundant bacterioplanktonic groups in oceans worldwide.[1,2,3] RCB are free-living, but they are o en found in epibiotic bio lms on macro-algae and various invertebrates and are key players in the carbon and sulfur cycles and have a variety of mechanisms for obtaining energy
Sagittula stellata is a heterotrophic member of the alpha proteobacteria Roseobacter clade typically found in marine environments responsible for the degradation of cellulose, lignin related compounds and for the oxidation of dimethylsul de (DMS) to dimethyl sulfoxide (DMSO) that is coupled to ATP synthesis and other organic sulfur compounds typically found in surface.[6,7]
The chemical composition of marine microorganisms have been monitored by FTIR spectroscopy providing unique insight for studying the bacteria dynamics
Summary
Marine Roseobacter clade bacteria (RCB) are one of the most abundant bacterioplanktonic groups in oceans worldwide.[1,2,3] RCB are free-living, but they are o en found in epibiotic bio lms on macro-algae and various invertebrates and are key players in the carbon and sulfur cycles and have a variety of mechanisms for obtaining energy. Sagittula stellata is a heterotrophic member of the alpha proteobacteria Roseobacter clade typically found in marine environments responsible for the degradation of cellulose, lignin related compounds and for the oxidation of dimethylsul de (DMS) to dimethyl sulfoxide (DMSO) that is coupled to ATP synthesis and other organic sulfur compounds typically found in surface.[6,7] Phototrophic bacteria found in marine environments use DMS which is a volatile organosulfur. Trace metals such as iron and copper play important roles in the ocean because they are essential for the growth of marine phytoplankton. To our best knowledge this is the rst application of a comprehensive spectroscopic study involving Raman, FTIR and uorescence spectroscopies for chemical analysis of the complex bacteria matrix formed by the metal ion binding to S. stellata illustrating the potential of the techniques to perform ultrasensitive chemical dynamics analysis of marine bacteria, including the detection of different components and the determination of their relative abundance with the metal ions Cu(II), Fe(III), Mg(II), and Zn(II)
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