Initial experimental multi-wavelength EEM (Excitation Emission Matrix) fluorescence lidar detection and classification of atmospheric pollen with potential applications toward real-time bioaerosols monitoring.

Abstract

Fluorescence has the potential to identify the types of substances associated with aerosols. To demonstrate its usefulness in environmental studies, we investigated the use of Excitation-Emission-Matrix (EEM) fluorescence in lidar bioaerosol monitoring. First, the EEM fluorescence of cedar, ragweed, and apple pollens as typical bioaerosols found around our surroundings were measured using a commercial fluorescence spectrometer. We found that the patterns of fluorescence changed depending on the pollen type and excitation wavelength and it meant that studying these EEM fluorescence patterns was a good parameter for identifying pollen types. Then, we setup a simple EEM fluorescence lidar to confirm the usefulness in lidar bioaerosol monitoring. The lidar consisted of three laser diodes and one light emitting diode with output at 520 nm, 445 nm, 405 nm and 325 nm, respectively, an ultra violet camera lens as a receiver, and a fluorescence spectrum detection unit. Comparing the lidar simulation results with the EEM fluorescence dataset supported the possibility of performing bioaerosol monitoring using the EEM fluorescence lidar. Based on the results and the current technology, a feasible design of a bioaerosol detection EEM fluorescence lidar is proposed for future rel-time remote sensing and mapping of atmospheric bioaerosols.