Abstract
PurposeThe study aimed to find out whether fluorescence parameters of humic acids (HA), obtained from excitation-emission matrix (EEM) fluorescence spectra, could be used to explain the origin and properties of lake sediments organic matter.Materials and methodsThe research material was HA extracted from lake sediments. Sediment samples were collected in the summer stagnation, from maximum depth of each lake. Humic acids extraction was performed using the method proposed by the International Humic Substances Society. In extracted HA, the elemental composition, free radical content, UV-Vis, and EEM fluorescence spectra were determined. EEM spectral modeling was performed using a multidirectional statistical method—parallel factor analysis PARAFAC.Results and discussionFour specific, two protein-like (C2 and C4) and humic- and fulvic-like (C1 and C3) components, components responsible for emission of fluorescence were identified in HA extracted from lake sediments. In the obtained EEM spectra, the dominant component was C2, while the smallest participation have C4 component. The mean contribution of C3 and C1 components to the total intensity of fluorescence was almost the same and was about 25%. Significant correlations were obtained between the concentration of EEM components and parameters characterizing the chemical properties of sediments and humic acids.ConclusionsThe study showed that the EEM fluorescence spectroscopy can be successfully used to characterize natural organic matter in aquatic environments. This method may provide supplementary information on the origin and transformation of organic matter in the water environments. By applying the parameters obtained from PARAFAC modeling, the relative degree of organic matter transformation (component C1) and autotrophic productivity (component C3) may be assessed. The analysis of the component C1 and C4 complete the information about the organic matter origin.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
