Abstract

In this study we tested the cellular uptake and the phototoxicity of meso-tetrakis(4-sulfonatophenyl)porphyrin (TPPS4) and its metallocomplex ZnTPPS4 in the presence or absence of 2-hydroxypropyl-cyclodextrins (hpCDs) on G361human melanoma cells. The dependence of concentration of porphyrin sensitizers in combination with laser irradiation on photodamage of G361 cells was investigated by in vitro methods. Viability of cells was determined by means of molecular probes for fluorescence microscopy. The quantitative changes of cell viability in relation to sensitizers concentrations and irradiation doses were proved by fluorometric measurement. G361 cells are sensitive to photodynamic damage for both of the tested sensitizers. TPPS4 and ZnTPPS4 especially in the supramolecular complex with nontoxic cyclodextrin carriers represent efficient sensitizers with high phototoxicity to G361 human melanoma cells.

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