Abstract
The goal of this work was to investigate the photodynamic activity of 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl]chlorin (TAPC) and zinc(II) 2,9,16,23-tetrakis[4-(N-methylpyridyloxy)]phthalocyanine iodide (ZnPPc4+ ) as photosensitizers to inactivate Staphylococcus aureus biofilms and prevent their formations in different culture media. We incubated Saureus biofilms in different culture media: tryptic soy (TS), nutrient (N), Müeller Hinton (MH) broth, TS with glucose 2 and 5% (w/v) with 5μM ZnPPc4+ or TAPC and irradiated with visible light (350-800nm). Photodynamic inactivation (PDI) was determined by count of colony forming units (CFU) and crystal violet method. Furthermore, we studied PDI effect on biofilm development in TS broth. Finally, we examined the effects of PDI on the structure of Saureus biofilm. Greater inactivation was achieved, using TAPC or ZnPPc4+ , when Saureus biofilm was grown in N or MH broths rather than in TS. Besides, glucose addition to the medium decreases the ability to develop biofilm and increase the photoinactivation capacity. Prevention of 3 log biofilm developments was obtained when Saureus cultures were treated with TAPC (10μM) and 108J/cm2 in TS broth and the number of CFU was counted after 24hours. Moreover, microscopy studies demonstrated modifications in biofilm architecture. These results indicate that TAPC and ZnPPc4+ may be promising photosensitizers for photodynamic inactivation of Saureus biofilms or to prevent their formation.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call