Abstract
OBJECTIVES: The purpose of this in vitro study was to compare the antimicrobial substantivity of 1% MTAD, 2% Chlorhexidine (CHX) and 1.3% sodium hypochlorite (NaOCl) in bovine root dentine. METHOD: One hundred and ten dentine tubes prepared from bovine incisors were infected in vitro for 14 days with Enterococcus faecalis. The specimens were divided in to five groups as follows: CHX; MTAD; and NaOCl; infected dentine tubes (positive control); and sterile dentine tubes (negative control). Dentine chips were collected with round burs into tryptic soy broth (TSB). After culturing the number of colony-forming units (CFU) was counted. RESULTS: In all experimental groups CFU was minimum in the first cultures. The results obtained were significantly different from each other at any time period (P<0.05). In the first culture, the NaOCI group and CHX group showed the lowest and highest number of CFU, respectively. In each group, the number of CFUs increased significantly by time-lapse (P<0.05). CONCLUSISON: In conclusion, the substantivity of MTAD was significantly greater than CHX and NaOCl.
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