Photoactivation of Autologous Transplantation of Subcutaneous Adipose Tissue Improves Glucose Homeostasis

Abstract

Photoactivation of autologous transplantation of subcutaneous adipose tissue improves glucose homeostasis Increasing evidences indicated that normal adipose tissue transplantation improves whole-body energy metabolism and glucose homeostasis in a high-fat diet (HFD)-induced obese mice model. Adipose tissue macrophage is associated with glucose homeostasis and insulin resistance in type 2 diabetes and obese humans, offering a potential target for therapeutics. However, whether transplantation of autologous AT that changed macrophage phenotype directly contribute to systemic glucose intolerance has not been determined. Here we specifically developed our device, with more specific wavelengths of light, to activate macrophage phenotype in isolated sWAT from HFD mice. We found that the macrophage polarization, M1 marker genes expression, such as CD11c, IL-6 and monocyte chemoattractant protein-1, but also the M1-to-M2 ratio were increased by an HFD and decreased by photoactivation treatment. Furthermore, autologous transplantation of photoactivated sWAT into a HFD mice reduced blood glucose level and caused significant improvement in glucose tolerance, which was not shown in a sham-operated or non-photoactivated sWAT HFD mice. Moreover, Positron emission/computed tomography scans indicated higher glucose uptake in heart, but not liver, hindlimb muscles, and abdominal subcutaneous white adipose tissue. These data suggested that the correlation with photoactivation to shift ATMs polarization of HFD mice caused significant improvement in glucose homeostasis, and that autologous transplantation might be a promising therapeutic option for the treatment of diabetes. Disclosure J. Wu: None.