Pharmacological studies of Selenium Nanoparticles synthesized from Syzygium aromaticum

Abstract

Selenium is an essential element and if taken in trace amounts, then it plays an important role in plant and animal development and reproduction. When compared to other methods of selenium nanoparticles (SeNPs) production, green synthesis using plant extracts has a better probability of eliminating its harmful character and producing beneficial SeNPs. The current work used Syzygium aromaticum to perform biogenic synthesis of SeNPs. The biosynthesized SeNPs were characterized by UV-Vis spectroscopy, X-Ray Diffraction, FTIR, AAS, SEM-EDX and FESEM. The biosynthesized SeNPs showed maximum peak at 276 nm and FESEM image exhibited greater numbers of the agglomerated spherical particles within the diameter range 30-55 nm. The antioxidant activity was measured in vitro using the radical scavenging (DPPH) test, total antioxidant (FRAP) assay and intracellular reactive oxygen species (ROS) assay against the A375 cell line. The formation of free radicals of selenium elements as antioxidant qualities prevented the growth of A375 skin cancer cells. In vitro tests revealed that SeNPs had the highest percentage of inhibition. As a consequence, the current study's findings showed that the lowest concentrations of SeNPs had better antioxidant effects. MTT experiment was used to assess the cytotoxicity of SeNPs against A375 cell lines exhibiting an IC50 value of 27±1.2 g/ mL. Synthesized SeNPs showed less toxicity towards the investigated cell line which remained more than 50% viability at 48 hours of exposure. In vitro anthelmintic activity data demonstrate that the worms experienced paralysis and death time at 25.03± 0.12 and 60.12±1.90 minutes respectively. These SeNPs with diverse objectives may exhibit increased absorption by functionalization and simultaneous drug loading as well as agnostic characteristics in the future.