Abstract
DNA fiber autoradiography and alkaline sucrose sedimentation of DNA of cultured pea-root cells ( Pisum sativum) arrested in G2 by carbohydrate starvation demonstrated that nascent DNA molecules of replicon (16–27 × 10 6 D) and apparent cluster (~330 × 10 6 D) size were not joined. That the arrested cells were in G2 was confirmed by single-cell autoradiography and cytophotometry. In pea there are about 18 replicons per average cluster, 4.2 × 10 3 clusters, and 7.7 × 10 4 replicons per genome.
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