Abstract
A strepzyme lytic to Verticillium albo-atrum mycelium was obtained from a wild Strep? tomyces isolate cultured from local soil. Peak strepzyme production in a liquid mineral medium was 5 da. Treatment of 1 -da-old V. albo-atrum mycelium with concentrated, desalted strepzyme stabilized in 0.7 m MgS04 yielded maximum (ca. 95%) protoplast release in 20 h. The sequence of protoplast release, observed by phase contrast microscopy, was similar to that reported for other fungi. Equal numbers of V. albo-atrum protoplasts or conidia introduced into root xylem of Fcrt/d///wra-susceptible eggplants, tomatoes, and yellow-poplar seedlings resulted in vascular disease in eggplants and tomatoes only. Typical V. albo-atrum mycelium was recovered from diseased tissue by culture on a Verticillium-selective medium.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
