Abstract
Aim. To evaluate the effectiveness of a consortium of hydrocarbon oxidizing bacteria in various model systems. Methods. Two types of model systems were used for carrying out the experiments: with a liquid mineral medium and with non-sterile soil. To determine the number of microorganisms, a standard method of serial dilutions with seeding to individual colonies was used. Individual strains in the consortium were distinguished using selective media with antibiotics. Oil degradation was assessed by IR spectrometry. Results . The consortium was capable to effective oil destruction in a liquid medium at 4°C and at 24°C, and with respect to control, oil loss is higher at low temperature. At 50°C, the consortium was inactive. In model non-sterile soil systems, the stimulation of native microorganisms by introducing mineral fertilizers did not lead to significant changes in the number of oil-degrading microorganisms but the degree of oil degradation increased. With the joint introduction of the microbial consortium and fertilization, the greatest number of both heterotrophic and oil-degrading strains was observed in the soil system. The degree of oil destruction in this system was also the highest: 59% at 42 days at room temperature. Main conclusions . The developed bacterial consortium has a high oil-degrading activity both at low (4°C) and moderate (18-25°C) temperatures. In non-sterile soil systems, the consortium's microorganisms do not inhibit the local biota, maintaining their numbers at about constant levels, but at the same time they make the main contribution to pollution degradation.
Highlights
Приготовленную таким образом бактериальную суспензию добавляли в дистиллированную воду, и затем воду с инокулятом вносили в почву
Conflict of interest The authors declare no conflict of interest
Summary
БИОДЕГРАДАЦИЯ НЕФТИ КОНСОРЦИУМОМ ШТАММОВ-НЕФТЕДЕСТРУКТОРОВ В ЛАБОРАТОРНЫХ МОДЕЛЬНЫХ СИСТЕМАХ. Оценить эффективность консорциума углеводородокисляющих бактерий в различных модельных системах. В модельных почвенных системах стимуляция аборигенных микроорганизмов путем внесения минеральных удобрений не приводила к значительным изменениям численности микроорганизмов-нефтедестукторов, однако степень деградации нефти увеличилась. При совместном внесении микробного консорциума и удобрения в почвенную систему наблюдалась наибольшая численность, как гетеротрофных бактерий, так и углеводородокисляющих штаммов. Степень деструкции нефти в этой системе также была наибольшей: 59% через 42 суток при комнатной температуре. В нестерильных почвенных системах микроорганизмы консорциума не подавляют местную биоту, сохраняя свою численность примерно на постоянном уровне, но при этом вносят основной вклад в деградацию загрязнения. Ключевые слова: бактериальный консорциум, биодеградация нефти, экологическая биотехнология, низкие температуры. Биодеградация нефти консорциумом штаммов-нефтедеструкторов в лабораторных модельных системах // Юг России: экология, развитие. OIL BIODEGRADATION BY CONSORTIUM OF OIL DEGRADING MICROORGANISMS IN LABORATORY MODEL SYSTEMS
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