Abstract
Since the cloned sheep “Dolly” was born in 1997, somatic cell nuclear transfer (SCNT) has been achieved in many species, however, to date, the overall cloning efficiency is still low, and this limits the large-scale application in basic research, agriculture and medicine, etc. It is generally believed that low cloning efficiency is mainly due to aberrant epigenetic reprogramming. In cloned embryos, DNA methylation, histone modifications and genomic imprinting, etc, are usually disrupted, and these incomplete epigenetic reprogramming causes continuous expression of tissue specific genes, no effective activation of genes related to early embryo development, and aberrant transcription of imprinted genes, etc, thereby leading to poor cloning efficiency.
Published Version
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