Abstract
<p class="western" style="margin-bottom: 0cm; line-height: 100%;" align="justify"> </p><p class="western" style="margin-bottom: 0cm; line-height: 100%;" align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US">The aim of the present study was to determine the level of ginsenosides in </span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US">extracts from </span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US">hairy root A, B, G clones of</span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US"><em> Panax quinquefolium </em></span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US">and their action with antibiotics against clinical bacterial isolates. The content of ginsenosides (the key biologically active compounds) were determined in tested extracts using HPLC. The activity of extracts with antibiotics was established by micro-dilution broth method. Total triterpene saponin content was 14.68, 14.32 and 10.07 mgg</span></span></span></span><span style="color: #000000;"><sup><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US">-1</span></span></span></sup></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US"> d.w. for root culture clones B, A and G, respectively. Our research indicates that the addition of extracts mainly from B and G clone hairy root cultures to antibiotics allow to reduce the ampicillin and tetracycline effective concentration respectively against </span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US"><em>Enterococcus faecalis</em></span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US"> and both </span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US"><em>Escherichia coli</em></span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US"> and </span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en-US"><em>Acintobacter baumannii</em></span></span></span></span><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en">.</span></span></span></span></p><p class="western" style="margin-bottom: 0cm; line-height: 100%;" align="justify"><span style="color: #000000;"><span style="font-family: Times New Roman, serif;"><span style="letter-spacing: -0.1pt;"><span lang="en"><br /></span></span></span></span></p>
Highlights
Ginsenosides are triterpene saponins isolated from various members of the genus Panax including Panax quinquefolium
In contrast to the earlier study on P. quinquefolium hairy roots cultivated in unmodiied B-5 medium [13], our present indings indicate that the age of the culture, expressed as the number of passages and composition of the medium, may inluence the total ginsenoside yield and the proile of individual metabolites
The total ginsenoside content of lines A, B, and G investigated in the present study was found to be approximately 14.68, 14.32, and 10.07 mg g-1 d.w. respectively
Summary
M and their effect in connection with antibiotics against pathogenic bacteria – Ua preliminary study. The aim of the present study was to determine the level of ginsenosides in extracts from hairy root A, B, G clones of Panax quinquefolium and their action with antibiotics against clinical bacterial isolates. The content of ginsenosides (the key biologically active compounds) were determined in tested extracts using hPLC. The activity of extracts with antibiotics was established by microdilution broth method. Total triterpene saponin content was 14.68, 14.32 and 10.07 mgg-1 d.w. for root culture clones A, B and G, respectively. Our research indicates that the addition of extracts mainly from B and G clone hairy root cultures to antibiotics allow to reduce the ampicillin and tetracycline effective concentration respectively against Enterococcus faecalis and both Escherichia coli and Acintobacter baumannii
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