Oxidative stress effects of fullerene-porphyrin derivatives in photodynamic therapy

Abstract

Due to their special and growing medical recent interest, the fullerenes started to be a very studied class of chemical compounds. In order to improve their water solubility and to reduce their cytotoxic characteristics, the fullerenes have been coupled in a system fullerene/PVP/porphyrin (C60/PVP/TPP) and its application in photodynamic therapy will be evaluated in this paper. The oxidative stress effects on photodynamic therapy with systems fullerene/poly-N-vinylpirrolidone/5,10,15,20-tetrakis(4-phenyl)porphyrin (C60/PVP/TPP) were tested on Wistar rats sub-cutaneously inoculated with Walker 256 carcinoma. The animals were irradiated with red light (λ = 685 nm; D = 50 J/cm2; 15 minutes) 24 h after intra-peritoneal administration of 10 mg/kg body weight of the system C60/PVP/TPP. After photodynamic therapy, the free radicals in tumors have been indirectly evaluated by lipid peroxides level (measured as thiobarbituric reactive substances) and protein carbonyls (indices of oxidative effects produced on susceptible biomolecules), both of them increasing in tumor tissues of animals 24 h after treatment. The levels of thiol groups and total antioxidant capacity have been determined in tumors, too, their decreasing values being the effect of the strong tumoral oxidative process.