Abstract
BackgroundInorganic polyphosphate bodies (PPB) have recently been linked to a variety of functions in mammalian cells. To improve the yield of PPB from Synechococcus sp. PCC 7002 and characterize its form, in this study, a recombinant plasmid containing a polyphosphate kinase (ppk) gene was generated and transformed into Synechococcus sp. PCC 7002.ResultsPPB separated by Sephadex G-100 was characterized and added to polarized human intestinal epithelial (Caco-2) cells, and the absorption effect was assessed. The ppk gene was stably expressed by induction with 1 μM nickel, and the resulting PPB yield from Synechococcus sp. PCC 7002 cells increased by 89.66%. Transmission electron microscopy and dynamic light scattering analyses showed that PPB from these cells were nanosized, ranging from a few to approximately 100 nanometres in diameter. PPB can be taken up by Caco-2 cells and are mainly distributed around lipid droplets.ConclusionsWe determined that PPB can be overproduced in Synechococcus sp. PCC 7002 and that the resulting PPB were well absorbed by Caco-2 cells. Microalgae provide a promising “cell factory” for PPB production.
Highlights
Inorganic polyphosphate bodies (PPB) have recently been linked to a variety of functions in mammalian cells
Unlike the short-chain polyP used in the food industry, microorganisms synthesize polyP can be hardly processed by alkaline phosphatase in mammalian intestines [15], so that it can be absorbed by the intestinal epithelial cells with endocytosis [16]
PCC 7002 was cloned using ppk primers by polymerase chain reaction (PCR) and the PCR products were observed by polyacrylamide gel electrophoresis (PAGE)
Summary
Inorganic polyphosphate bodies (PPB) have recently been linked to a variety of functions in mammalian cells. PCC 7002 and characterize its form, in this study, a recombinant plasmid containing a polyphosphate kinase (ppk) gene was generated and transformed into Synechococcus sp. Inorganic polyphosphate (polyP) is a linear polymer composed of three to several hundred phosphate groups that is found ubiquitously [7]. PPB ( known as volutin granules) are mineralized structures present in many cells and contain large amounts of polyP [10]. As a carrier of mineral elements [19], such as calcium [20], iron [21] and zinc [22], it is possible to develop PPB as a new mineral nutrition enhancer; Gao et al Microb Cell Fact (2018) 17:27 there are no relevant reports on this subject at present
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