Abstract
Sitobion miscanthi is awheat aphidspecies that can damage seriously agricultural production. The effective management of wheat aphids has depended on chemical insecticides. However, their wide application led to severe resistance of wheat aphids to some insecticides, and cytochrome P450 as a detoxifying enzyme plays a crucial role in the insecticide resistance. In this study, CYP6CY1, a new P450 gene, was isolated and overexpressed in a S.miscanthi resistant strain to imidacloprid. The increased sensitivity to imidacloprid after silencing of CYP6CY1 indicated that its involvement in imidacloprid resistance. Subsequently, action ofmiR-3047-3pwithin the 3' UTR of theCYP6CY1 was confirmed at the posttranscriptional regulatory level, and was shown to beinvolved in the resistance to imidacloprid. These results provide opportunities for understanding the roles of P450 in insecticide resistance of pests.
Published Version
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